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Re: [ccp4bb]: Cys modification
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If your cysteine gets 'ears' (i.e. density on both sides of
the sulfur at a distance of about 1 A) that could be a sign
of anisotropic mobility of the sulfur. This could potentially
show up at 1.7 A already.
> I've also been wondering about extra density that I see near the two
> cysteines in the model I'm working on. The data are up to 1.7A and were
> collected at 100K. Replacing the SH with SO3 does not fit well, in
> either case. The extra density is to one side of the sulfur and I
> currently have it modeled as a magnesium ion. Water doesn't fit well
> either, as it can't get close enough.
> Is this common? I checked a sub set of the pdb and did not find any
> metal ions near cysteines.
> Jeff Taylor
> Dr. Jeffrey S. Taylor Duke University Medical Center
> Department of Biochemistry Box 3711, Nanaline Duke Bld
> Phone (919) 681-5266 Durham, NC 27710
Manfred S. Weiss, Dr.
Institute of Molecular Biotechnology
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