Re: [ccp4bb]: Ammonium selenate for Se labelling of proteins

[Martyn Simmons <martyn@cryst.bioc.cam.ac.uk>]

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Dear Allan
          - I have been surprised at how well the metabolic inhibition
method of Van Duyne et al. works for E.coli. 
          - original ref was Van Duyne, G. D. et al. (1993) J.Mol Biol. 
vol. 229 pp105-124
          - this is a list of other papers that I think have used it.  The
basic idea is that you add a concentrated cocktail of amino-acids that
share that same biosynthetic pathway as Methionine and these naturally
down-regulate Methionine synthesis just before you add SeMet. So you don't
need a Met- strain.
          - I have a detailed protocol that worked for me (which you are
welcome to) but it is mostly just what I understood Van Duyne et al. to
have done. 
          - regards
                   Martyn

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