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Re: [ccp4bb]: refolding from inclusion bodies

For outer membrane protein refolding see Buchanan S. K., Current Opinion in Structural Biology 9:455-461 (1999) and references therein.


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I wonder which protocols have been succesfully used to refold proteins from
inclusion bodies for the purpose of crystallization. Can you point me to
specific references. Thank you very much,
                                              Menachem Shoham

          "Structures are only as good as the meaning they transmit"

Menachem Shoham, Ph.D.
Case Western Reserve University
School of Medicine, Department of Biochemistry
Rm. W509
10900 Euclid Ave
Cleveland, Ohio 44106-4935

Tel. 216-368-4665
Fax  216-368-3419

Arjan Snijder
Lab. of Biophysical Chemistry
University of Groningen
Nyenborgh 4
9747 AG Groningen
tel +31 (0) 50 3634396
fax +31 (0) 50 3634800