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[ccp4bb]: Asn-NAG bond

Dear All,

Sorry this is not a CCP4-related question:

While refining my structure, I can see density for a N-acetylglucosamine group at an Asparagine residue
(which confirms experimental data).  Using the topology and parameter files for a NAG group,  CNS
does not know yet that there is a bond between the NAG C1 atom and the Asn ND2 atom.  As a consequence,
CNS shifts the NAG residue away from the Asn.
What should I change in the topology / parameter files ?


Filip Van Petegem


Filip Van Petegem                 
Laboratory for Protein Biochemistry and Protein Engineering
Department of Biochemistry Physiology and Microbiology
K.L. Ledeganckstraat 35                Tel: (32)-9-264-5127
BP 9000 GENT                           Fax: (32)-9-264-5338
BELGIUM                 E-mail: Filip.VanPetegem@.rug.ac.be