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Re: [ccp4bb]: a crystal moubting problem
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How were you mounting the crystals? In a capillary or in a cryo-loop? If
the latter, have a careful read-through of Garman and Schneider (1997), J.
Appl. Crystallography 30, pp. 211-237. And probably other papers too.
Important would be to try out various cryo-soaking protocolls.
> sorry for this is not a ccp4 problem, but I know many people
> here have experience on it.
> I growed crystals unsing Ammonium Sulfate as precipitant, and
> the condition contains 10% Isopropanol. When mounting the
> crystals, the crystals cracked quickly. When I displayed the
> iospropanol with other organic reagents, crystala woundn't
> appear. So, any suggestions to mount the crystals?
> Thank you.
> Rong-Jin Guan