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Re: [ccp4bb]: methionine auxotroph strain

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Here is a link from Novagen that will get you to B834(DE3) competent cells, the
methionine auxotroph strain.
They offer both as B834(DE3) and as B834(DE3)pLysS (to minimize leaky expression);
I've had better luck with B834(DE3) but I'm sure it's protein-dependent, so it's wise
to try both and compare.

good luck

David R. Buckler, Ph.D.
679 Hoes Lane
Piscataway, NJ  08854
phone:  (732) 235-5164
fax:    (732) 235-5289
e-mail:  buckler@cabm.rutgers.edu