[ccp4bb]: maps and reflections

[king lau <liaojt@moon.ibp.ac.cn>]

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Dear All,

I'm a new user of O and attemping to modeling my structure with electron density maps(2fo-fc, fo-fc), but i don't know which level will be appropriate.(In Turbo Frodo,
it should be appropriate with level=1 for 2fo-fc map and level=2 for fo-fc map after sigma scaling ). Another question is that if we make another set of cross validating reflections at the middle stage of structure determination, how can i make it 'free' again? Can simulated annealing method make it truly 'free' again?

Does anyone have good suggestions?

king lau

liaojt@moon.ibp.ac.cn