[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]

Re: [ccp4bb]: non-specifically bound heavy atom?



***  For details on how to be removed from this list visit the  ***
***    CCP4 home page http://www.dl.ac.uk/CCP/CCP4/main.html    ***

On Fri, 15 Sep 2000, Rui Zhao wrote:

> I have soaked a crystal in 1mM ethylmercury phosphate for 2 days
> (There is 1 cys in the 38kD protein.)

did you do a 'back-soak' afterwards to remove excess or loosely bound
mercury from the solvent space?  a) doing so could help clean up the
Riso.  b) if so, an X-ray fluorescent scan, which is routine before a MAD
experiment, should tell you if you still have any mercury in your
crystals.

=======================================================================
"I am an omnivorous reader with a strangely retentive memory for trifles"
                                                      -- Sherlock Holmes
=======================================================================
                        David J. Schuller
                        modern man in a post-modern world
                        University of California-Irvine
                        schuller@uci.edu