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Re: [ccp4bb]: non-specifically bound heavy atom?

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On Fri, 15 Sep 2000, Rui Zhao wrote:

> I have soaked a crystal in 1mM ethylmercury phosphate for 2 days
> (There is 1 cys in the 38kD protein.)

did you do a 'back-soak' afterwards to remove excess or loosely bound
mercury from the solvent space?  a) doing so could help clean up the
Riso.  b) if so, an X-ray fluorescent scan, which is routine before a MAD
experiment, should tell you if you still have any mercury in your

"I am an omnivorous reader with a strangely retentive memory for trifles"
                                                      -- Sherlock Holmes
                        David J. Schuller
                        modern man in a post-modern world
                        University of California-Irvine