Re: [ccp4bb]: removeing ncs

["Eleanor J. Dodson" <ccp4@ysbl.york.ac.uk>]

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Leonard Thomas wrote:
> 
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> Hi all,
> 
> Just a simiple (I think) question about using NCS restraints.  I have a
> structure that has 2 mol/asymm.  I have been using NCS retraints sofar
> with  pretty good results.  My question is when do most people remove
> the restraints, if they are removed at all. The compound is not a dimer
> so there must be some difference in the molecules.
> 
> Thank you for your comments.
> Len
>   
> --
> Leonard M. Thomas Ph.D.                 Phone (816)235-2571
> University of Missouri - Kansas City    Fax   (816)235-1503
> School of Biological Sciences           email thomasle@umkc.edu
> 5100 Rockhill Road, Kansas City, MO 64110


 Depends on the resolution of your data - at 3A - wouldnt release at
all;
at 2.0, when the Rfactors have converged..
 You should see differences in the maps then I think
Eleanor
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Eleanor J.Dodson, Chemistry Department, University of York, U.K.
Tel: Home +44 (1904) 42 44 49, work:  +44 (1904) 43 25 65
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