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Re: [ccp4bb]: summary for NCS averaging
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Jinsong Liu wrote:
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thanks for the nice summary..
> PS: one last question, just a little doubt, will that translation NCS
> prevent me finding the Se sites? I don't think so, but we should
> have 9 sites per protein instead of one. No matter how hard I tried, I
> can only find one Se per protein (so two sites per ASU), the
> two Se sites are related by (0, 0.04,0.5).
This is bad news; translational symmetry might make it harder - more
complicated pattersons etc, but if you have only found one Se there is
something seriously wrong - possibly no Se in the crystal; possibly not
How did you position the Se ? If the MR solution is correct, phases
based on the solution should be able to show you the Se sites; I usually
do a Dano fourier, but a dispersive difference fourier should show you
the same sites.