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[ccp4bb]: Re: your mail
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Break your searching model into two models. Then do MR search for each
of them. If you use epmr, search for the 4 copies of domain 1 first.
Check the packing (symmetry mates) of your solutions. If it looks
reasonable, take the solution, do epmr again to search for the 4 copies of
domain 2. You may experience difficulty to find the 3rd or 4th copy of
your search domain, try sigmaa in CCP4. Look at the sigmaa-weighted 2Fo-Fc
and Fo-Fc maps. You have good chances to locate the missing ones. Along
the way, always be aware the validation of your solutions ( you expect 8
solutions) by checking the packing and consistancy with your NCS symmtry
revealed by self-rotation. Good luck.
Xiaojing Yang, Ph.D.
The University of Chicago
email. yang@vishnu.uchicago.edu
- References:
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- From: Palmira daSilva <dasilva@scripps.edu>