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[ccp4bb]: crystallization of oxygen-sensitive sample

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this is a question to those readers with experinece in the crystallization oxygen-sensitive proteins.

I assume that I will have to remove dissolved oxygen in the well solutions.

What is the best way of degassing 50+ different solutions for my initial screening?

Might it be enough to incubate open tubes for a while in an inert atmosphere or is bubbling-through of inert gas required?

Any thoughts on this you may wish to share are appreciated.

Many thanks in anticipation.

Wolfram Tempel

Life Sciences Building B206
The University of Georgia
Athens, GA 30602

Phone:  +1 (706) 542-3403
Fax:    +1 (706) 542-3077