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Re: Freezing
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HI Swami,
what about trying freezing crystals in some other salt like sodium format?
There is a paper published where the authors reported freezing crystals in
6 M sodium format (crystals grew from 3.5 M sodium format; Lee et al
(1998), Nature 391:859-865).
Sincerely,
Katjusa
*****************************
Katjusa Brejc
Netherlands Cancer Institute
Dept. Molecular Carcinogenesis
Plesmanlaan 121
1066 CX AMSTERDAM
The Netherlands
*****************************
> Dear :
>
> I have got a crystal and the condition is:
>
> 100 mM Tris-HCl, pH 7.5
> 150 mM Nacl
> 20 mM Ca Cl2
>
> (The crystal dissolves in conventional method of freezing, time course
> transfer in artificial mother liquor + 5%, 10%, 15%, 20%, 25% glycerol, MPD,
> PEG400). I am looking for suggestions/advice for freezing crystals grown in
> pure salt environment. Any tips?
>
> With regards,
> Swami
>
> ****************************************************************************
> Dr. Kunchithapadam Swaminathan Phone: (65) 873 2173 (H)
> Institute of Molecular Agrobiology (65) 872 7408 (W)
> The National University of Singapore Fax: (65) 872 7007 (W)
> 1 Research Link, Singapore 117604 Email: nathan@ima.org.sg
>
> Web: www.ima.org.sg/research/struct_bio/struct_bio.html
> ****************************************************************************
>
>