protein solution

[Lan Zhou <lzhou2@emory.edu>]

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Dear Experts:

Pardon me for asking a non-CCP4 related question here.  But, I really feel 
somebody out there can help me solve my problem.

I have a very-well expressed protein.  It is fine at both 4c and room 
temperature (rt) as long as its concentration is below 1 mg/ml.  If I 
concentrate the protein to 4 mg/ml or even 20 mg/ml or so, they are still 
fine at 4c.  However, the protein solutions become very cloudy if I
transfer them to rt or 16c.  The protein solutions will turn to clear
again after they are put back to 4c for a while.

Is there anyone who has experience this before ?  What could be the
problem ?  Does it mean something wrong with the protein folding ? Is
there any suggestion ?

Your help is really appreciated.  Thanks in advance.

Lan Zhou