Re: Temperature factors and Wilson Plots

[Yu Wai Chen <ywc@mrc-lmb.cam.ac.uk>]

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> As yet, this all seems unremarkable.  What surprised me, however, was
> that the
> temperature factors for the refined structure were quite high.  They
> average temperature
> factor is 65 and ranges from 40 to 130.  I browsed through the old posts
> to
> this list and found a suggestion that your protein atom temperature
> factors should
> approximate your B from a Wilson plot.  I then looked at my truncate
> output
> and found the Wilson B value was 56.

To comfort you, here are my figures of a recent structure solved, which
is very similar to yours:

data to 2.5 A
R/R-free = 28/34
average B = 62
B estimated from Wilson = 59

Wai
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Yu Wai CHEN, Ph.D. ..................   email:ywc@mrc-lmb.cam.ac.uk
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