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[ccp4bb]: soluble aggregation of proteins

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Dear all,

We are expressing proteins here in E. Coli in the pMALp2 vector as
maltose-binding fusion proteins. This is a periplasmic expression and we
have found that our three proteins in the 22-38 KDa range all aggregate
in solution. 

Any suggestions from your experience to treat this type of problem?

Thanks,

Janet.

-- 
Janet Moloney, Division of Structural Biology,
Wellcome Trust Centre for Human Genetics,
University of Oxford, Roosevelt Drive, 
Oxford OX3 7BN, U.K.

Tel: 0044(0)1865 287551	   Fax: 0044(0)1865 287547