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Re: [ccp4bb]: refolding from inclusion bodies
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I have found this protocol on the web and it worked to produce active
enzyme from inclusion bodies:
http://www.path.cam.ac.uk/~lv213/protocol2.html
The only change I made was instead of 1M NDSB I used .25M. Good luck
Jose
On Thu, 29 Jun 2000, Menachem Shoham wrote:
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> *** CCP4 home page http://www.dl.ac.uk/CCP/CCP4/main.html ***
>
> I wonder which protocols have been succesfully used to refold proteins from
> inclusion bodies for the purpose of crystallization. Can you point me to
> specific references. Thnak you very much,
> Menachem Shoham
>
> "Structures are only as good as the meaning they transmit"
>
> Menachem Shoham, Ph.D.
> Case Western Reserve University
> School of Medicine, Department of Biochemistry
> Rm. W509
> 10900 Euclid Ave
> Cleveland, Ohio 44106-4935
> U.S.A.
>
> Tel. 216-368-4665
> Fax 216-368-3419
>
>
>
Jose Trincao
Physiology & Biophysics
1425 Madison Av., room 16-26, box 1677
New York, NY 10029
USA
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