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[ccp4bb]: RE: about the crystal mounting problem
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Dear all:
I am sorry having not expressed my problem clearly.
I growed crystals using 2.0 M ammonium sulfate and
the researvoir contained 10% isopropanol. The crystals
proved protein ones, after dyed with the Hampton Izit.
When I mount them using a capillary, it soon cracked.
Now I have read many replies,from which I got some
suggestions:
1. using dome kinds of oils to control the evaperation
of isopropanol
2. after the crystals growed,change the isopropanol to
other less volatile organic solvent
3. mount the crystals at a microenvironment with high
relative "humidity" in ipropanol
4. try and mount in a cold-room at 4C
5.Add more i-Pr alcohol to your drop, before and during
crystal manipulation
Thanks for the suggestions.
Rong-Jin Guan
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Rong-Jin Guan
grj@pewdc.ibp.ac.cn
guanrj@chinaren.com
Protein Engineering Laboratory
Institute of Biophysics
Chinese Academy of Sciences
Beijing 100101
Tel:(010) 64888548(lab)
(010) 64886847(room)
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