[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]

Re: [ccp4bb]: a question on crystallization



***  For details on how to be removed from this list visit the  ***
***    CCP4 home page http://www.dl.ac.uk/CCP/CCP4/main.html    ***

On Fri, 29 Dec 2000, Masato Kikkawa wrote:

> Sometimes, I can find 'oil drops' in the
> hanging drops.

you are seeing a separation into 2 liquid phases. you could try adding
organic additives to reunite the phases. (detergents, SDS, MPD,
hexanediol, dioxane, etc, typically at 0.1 - 5.0%)

=======================================================================
"Writing is a lonely, silent and difficult existence and I don't feel I
have the ideas, the imagination or the patience any more." - Colin Dexter
=======================================================================
                        David J. Schuller
                        modern man in a post-modern world
                        MacCHESS, Cornell University
                        schuller@uci.edu