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[ccp4bb]: crystallization of oxygen-sensitive sample
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Hi,
this is a question to those readers with experinece in the crystallization oxygen-sensitive proteins.
I assume that I will have to remove dissolved oxygen in the well solutions.
What is the best way of degassing 50+ different solutions for my initial screening?
Might it be enough to incubate open tubes for a while in an inert atmosphere or is bubbling-through of inert gas required?
Any thoughts on this you may wish to share are appreciated.
Many thanks in anticipation.
--
Wolfram Tempel
Life Sciences Building B206
The University of Georgia
Athens, GA 30602
Phone: +1 (706) 542-3403
Fax: +1 (706) 542-3077