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Re: [ccp4bb]: disulfide isomerase and refolding!
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>Have people succeded in using PDI to successfuly refold proteins
>from e.coli overexpressed mixed disulfide scenario.
Have a look at:
Martens E, Alloza I, Scott CJ, Billiau A, Vandenbroeck K
Eur J Biochem 2000 Nov;267(22):6679-83
Protein disulfide isomerase-mediated cell-free assembly of
recombinant interleukin-12 p40 homodimers.
Mayer M, Kies U, Kammermeier R, Buchner J
J Biol Chem 2000 Sep 22;275(38):29421-5
BiP and PDI cooperate in the oxidative folding of antibodies in vitro.
van den Berg B, Chung EW, Robinson CV, Mateo PL, Dobson CM
EMBO J 1999 Sep 1;18(17):4794-803
The oxidative refolding of hen lysozyme and its catalysis by protein
disulfide isomerase.
Hope this helps,
Luca
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Luca Jovine, Ph.D.
Department of Biochemistry and Molecular Biology
Mount Sinai School of Medicine
Annenberg Building, Room 25-18
One Gustave L. Levy Place, New York, NY 10029-6574, U.S.A.
Voice: +1.212.241-8620 FAX: +1.212.831-7126 eFAX: +1.509.356-2832
E-Mail: jovinl02@doc.mssm.edu W3: http://www.mssm.edu/students/jovinl02
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