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Re: [ccp4bb]: Missing AA in MAD map
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Kunchithapadam Swaminathan wrote:
>
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> Dear:
>
> I do not see about 25 (of 176) n-terminal and 5 c-terminal amino acids in
> the MAD density map (totalling about 17%, 2.3Ang data, 6 Met).
>
> 1. What is your experience in the highest percentage of chain untraced in
> MAD map?
> 2. How much from question-1 was recovered (seen later) either using better
> native data or after refinement?
>
> With regards,
> Swami
How long is a piece of string!
Many many structures have randomly ordered stretches of variable
length, and that will mean you cant trace the change whatever phasing
method you have used..
Eleanor