Re: [ccp4bb]: DM - NCS averaging

["David J. Schuller" <djs63@cornell.edu>]

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On Wed, 7 Nov 2001, Ricardo Aparicio wrote:

> 2) It seems to be wise to take advantage of NCS BEFORE doing solvent
> flattening, in other words, to make dozens of AVER cycles before use of
> solvent flattening option. Is this correct?

no.
both averaging and solvent flattening (correctly done) reduce noise and 
improve maps.  since they work on different principles, they are more 
powerful together than separately.  it would be wise to use them both, 
along with histogram matching, throughout phase refinement, especially if 
you are also extending the resolution of your phase set.

i cannot recall anyone in the history of NCS averaging recommending 
averaging to the exclusion of solvent flattening.

=======================================================================
"He was pinched perspiringly in the epistemological dilemma of the skeptic,
unable to accept solutions to problems he was unwilling to dismiss as
unsolvable." - Joseph Heller
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                        David J. Schuller
                        modern man in a post-modern world
                        MacCHESS, Cornell University
                        djs63@cornell.edu