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Re: [ccp4bb]: Xtals come out on the skin of drop
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Hello,
I have the same situation at hand and indeed, oxidation can be one of
the reasons. What I find that can help is crystallization in a gel
or under oil.
In addition, I retrieve the crystals that are stuck to the skin
by using cryo loops. First, I make a hole in the skin away from any
crystal. Second, with the loop go underneath the skin and 'loop' the crystal
of the skin. This works just fine.
Jeroen.
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Jeroen Raymundus Mesters, Ph.D. Alias Geronimo
Dept. of Structural Biology and Crystallography, Institute of Molecular
Biotechnology, P.O.Box 100 813, D-07708 Jena, Germany.
Tel: +49-3641-656063, Fax: +49-3641-656062, E-mail: jmesters@imb-jena.de
http://www.imb-jena.de/www_sbx/home.html
--
If you can look into the seeds of time and say
which grain will grow and which will not - speak then to me (Macbeth)
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