[ccp4bb]: Questions about SCALA

[Jianghai Zhu <zhu5@purdue.edu>]

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Dear All,

I am trying to use SCALA to process my MAD datasets. After I finished the 
manual, I came up several questions.

In a MAD experiment, I have six deferent datasets. (3 wavelength, I+ and I- 
for each wavelength). The deference between I+ and I- is that PHI rotates 
from 0 to 180 degree. I integrated these six datasets in MOSFLM. In SCALA, 
should I use 3 RUNs (one for each wavelength) or 6 RUNs (seperate I+ and 
I-)? I understand that all those batchs in one RUN will be scaled to one 
level. But will all those deferent RUNs be scaled to the same level? I 
mean, besides those internal scale factors in one RUN, is there any 
external scale factors between RUNs? When I read the example for MAD in the 
manual, there is one thing I couldn't figure out. What is the purpose to 
use SCALEIT at the final step? Is there anything SCALEIT can do, but SCALA 
can not do? If the example wants to get the statistics, it didn't use the 
ANALYSE keyword in SCALEIT. Instead of that, it used REFINE ANISOTROPIC.

Thanks.

Jianghai


================================
Jianghai Zhu
Biochemistry & Molecular Biology
B203, Lilly Hall
Purdue University
Tel: 765-4949247 (O)
      765-4630228 (H)
================================