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Re: [ccp4bb]: refmac and prior phase information



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sameeta bilgrami wrote:
> 
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> 
> dear all,
>       after we run refmac with "prior phase
> information", what are the phases that we should use
> to calculate the final map ....should they be the
> usual PHWT or PHCOMB ....


 Yes - these are all COMBINED phases - combining the calculated phase
from the model, and the experimental phase information. You should use
FWT PHWT to get a 
2mFoDFc map. 
The PHCOMB would only be used if you needed to use an overall phase for
a heavy atom difference map or some non-standard purpose.

>       i have a protein in spacegroup p6522 with a very
> high solvent content .... i ran dm for solvent
> flattening and the density improved
> considerably.....what is the best way of using this
> improved density after model building ....do i run
> refmac with  "prior phase information" and use PHCOMB
> or that is wrong ...
>                     thanking you,
>                             Sameeta


 Your "prior phase information" for REFMAC5 should be PHIDM FOMDM or the
HL coefficints from DM.

You may need to "blur" this phase information by scaling down the FOMs - 
Try phase blur 0.7 

Then again look at a map with  FWT/PHWT 

Best wishes Eleanor