Re: [ccp4bb]: Oils with Conventional Mounts

[Richard Gillilan <reg8@cornell.edu>]

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> 
> On Tue, 30 Apr 2002, James Stroud wrote:
> 
> > Has anyone used oil to stabilize otherwise labile crystals for a
> > conventional room temperature mount?
>

I have collected data with lysozyme using room 
temperature oil vs conventional capillary on the
same beamline and get some disturbing results. 
I am fairly sure the crystals did not slip since
they were large. Capillary crystals survived many
1 sec exposures on our hot A1 beamline while 
oil-mounted crystals required attenuation and 
even then died after just a couple exposures. 
I would be interested to hear if anyone else
has tried this. I'm guessing the oil does not
dissipate heat well (it is an insulator after all).
Obviously it is not a problem at LN2 temps.


 Richard Gillilan
 MacCHESS
 Cornell