[ccp4bb]: Re: Xtals

[Daniel Schlieper <daniel.schlieper@hollinux.org>]

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Dear Jan,

"Drying drops" worked fine for me. I used sitting drops in Cryschem/VDX
plates (24 wells), sealed with crystal clear tape. With a needle, I
punced a hole into the tape of each well. Maybe that changed the kinetics
of the crystal growth, rather than the thermodynamics. However, using an
open system instead of a closed one made the difference between needles
and nice twinned crystals :-)

Regards, Daniel

--
Daniel Schlieper               MRC Laboratory of Molecular Biology
daniel@mrc-lmb.cam.ac.uk       Hills Road, Cambridge CB2 2QH, UK
Tel.: +44 1223 252969          FAX: +44 1223 213556
WWW: http://www2.mrc-lmb.cam.ac.uk/groups/JYL/index.html

On Mon, 13 May 2002, Jan Dohnalek wrote:

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>
> Hi,
> I am desparately trying to grow 'thicker' crystals of my 'needles'.
> Has anyone had experience of getting this done. pH, protein
> concentration,
> drop size and temperature do not help. Some dependance on additives but
> nothing great.
> Any useful ideas?????
> (Yes, seeding also did not change the size)
> Jan
>