Re: [ccp4bb]: glutaraldehyde treatment of Xtals

[Arjan Snijder <arjan.snijder@molbiotech.chalmers.se>]

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Hi Ursula,

A good one to start with is Lusty, Journal of Applied Crystallography 1999
32,p106-112

briefly crystals are cross-linked using vapour diffusion. A hanging drop
is placed over a sitting drop setup with crystallisation solution or
stabilising solution. A 1 % glutaraldehyde solution is applied as a
sitting droplet. Crosslink for an hour or so.

Good luck

Arjan


> ***  For details on how to be removed from this list visit the  ***
> ***          CCP4 home page http://www.ccp4.ac.uk         ***
>
> Does somebody have experience with crosslinking Xtals with
> glutaraldehyde? I would be grateful if you could give me some protocol,
> reference,  or concentration ranges to start with.
>
> Thanks
>
> Ursula Schulze-Gahmen
> LBNL
> Berkeley, CA 94720