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Re: [ccp4bb]: A SAD case...



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On Wed, 24 Jul 2002, OnLineHelpForm wrote:

> DM). Even better, the map is fairly interpretable, and RESOLVE can trace
> almost 500f the protein as a polyalanine model.

OK, so let's assume your phases are reasonable.

> But, when I inspect the
> fom-weighte! d maps, all of the Se's are in the solvent!

Do you mean codisplay the Se *coordinates* with the map ?   If so I'd
suggest your Se coordinates are wrong, for whatever reason.  Just
calculate an anomalous difference map phased with your DM phases, which
will usually show quite unambiguously where the Se actually are, which is
probably not in the solvent....  You can also peak-pick the map (e.g. with
PEAKMAX) to get a correct set of Se coordinates.


Phil
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| Phil Jeffrey                                  |                             |
| Crystallography Facility Manager              | If you lie to the compiler, |
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