[ccp4bb]: Dithionite in Electron density maps

["Francisco J. Enguita" <fenguita@itqb.unl.pt>]

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Dear all :

I am dealing with the refinement of a protein structure reduced with
excess of dithionite. I reduced directly the crystals until a colour
change was observed, by soaking them in a dithionite solution.

I am able to see some positive density in the difference maps which
could correspond either to dithionite or its degradation product after
oxidation. 

Is/was someone dealing with a similar problem ?. How common is to see
dithionite in electron density maps after protein reduction ?.

Best regards and thanks in advance

Francisco



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Francisco J. Enguita, Ph.D.
Protein Crystallography laboratory
Instituto de Tecnologia Química e Biológica
Ap. 127
2781-901 Oeiras
PORTUGAL
Phone : 351-21-4469669
Fax : 351-21-4411277
E-mail : fenguita@itqb.unl.pt

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