[ccp4bb]: non-staining proteins??

[Neil Isaacs <neil@chem.gla.ac.uk>]

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We have a detergent (DDM) solubilised membrane protein with an HA flag
attached. Western blots with 2 different anti-HA antibodies clearly show
the protein. However, when the blot is overlaid on the SDS PAGE gel, there
is no match to the coomassie or silver-stained protein bands which appear
at slightly higher MWs. The MW markers however do overlap. We can't
explain this discrepancy (unless we assume the HA-protein is not taking up
stain). Has anyone had a similar experience and/or able to suggest a
reason?

Thanks

 Neil

 ________________________________________________________________
| Neil Isaacs                       Tel: +44 (0)141 330 5954     |
| Department of Chemistry           FAX: +44 (0)141 330 4888     |
| University of Glasgow           email: neil@chem.gla.ac.uk     |
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