Re: [ccp4bb]: High and low B-factors

[Marko Hyvonen <marko@cryst.bioc.cam.ac.uk>]

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On Thu, 28 Nov 2002, Artem Evdokimov wrote:

> > Are you sure you have 100 % Se-Met incorporation ? I would guess that if
> > part of your seleniums are in fact sulfurs, modelling the as seleniums
> > only would cause exactly what you describe now.
> >
> > Marko
>
> With all respect, I  disagree. Low incorporation won't make CE B-factor go
> lower. To me this looks like a wrong conformation of Met, leading to swap
> between CE and SE.
>
> A.G.E.
>


Aah, should have a coffee before reading emails. I only looked at the
second MSE data, with 10 A^2 higher B for Se compared to rest of the
sidechain (and I was mainly thinking of higher B of Se). My guess would
certainly not apply to the first case, and given the mass-spec data, not
to the second one either.

Marko