Re: [ccp4bb]: about fragile crystals

[William Scott <wgscott@chemistry.ucsc.edu>]

***  For details on how to be removed from this list visit the  ***
***          CCP4 home page http://www.ccp4.ac.uk         ***

One option is to try growing crystals in a gel.  Hampton Research I 
think sells a kit to do this, and I think some crystallization books 
describe it as well.  Also you can try adding a little bit of glycerol 
or other viscous solution to see if that helps to strengthen it.  
Finally, how are you trying to deal with it?  It is actually a lot 
easer to manipulate crystals with freezing loops than say glass 
capillaries.  Is it stuck to a surface?  You could try siliconizing 
cover-glasses for hanging drops, and I have even heard of people 
growing crystals on top of a layer of vacuum grease.


Bill Scott


On Friday, December 6, 2002, at 10:07 AM, xinqi liu wrote:

> ***  For details on how to be removed from this list visit the  ***
> ***          CCP4 home page http://www.ccp4.ac.uk         ***
>
> Hi, friends,
>
>   My crystal is too fragile. Every time when I tried
> to manipulate it, it became yellow color from clear
> and the packing is damaged. Anyone has good idea to
> deal with it? Thanks a lot.
>
> Sincerely,
> xinqi liu
>
>
> __________________________________________________
> Do you Yahoo!?
> Yahoo! Mail Plus - Powerful. Affordable. Sign up now.
> http://mailplus.yahoo.com
>
>