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Re: [ccp4bb]: substrate soaking into crystals

A protocol has been worked out in our lab in the late eighties,
where crystals grown in high ammoniumsulphate concentration
2.4 M) were transferred to various other precipitant solutions
with the same water activity to keep them stable. After
transferring to PEG 6000 the crystals could be used successfully
for soaking studies, because the sulphate ion previously bound
in the active site was now absent.
See :
Appl. Cryst. (1988). 21, 426-429    [DOI 10.1107/S0021889888004108]

The transfer of protein crystals from their original mother liquor to a solution with a completely different precipitant

H. A. Schreuder, H. Groendijk, J. M. van der Laan and R. K. Wierenga

Good luck,
Ing. Tjaard Pijning
Protein Crystallography Group
Department of Chemistry
University of Groningen
Nijenborgh 4, 9747AG Groningen
The Netherlands

(31)(0)50 363 4385  |   t.pijning@chem.rug.nl  | http://www.xray.chem.rug.nl/tjaard