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[ccp4bb]: NAD-binding Proteins



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I now that this is not a CCP4 question, but I need some help/advice with 
regard to Affinity Chromatography of an NAD binding protein.

I am using Bio-Rad's AffiBlue gel but am unable to get the protein to bind to 
the column.  Extensive dialysis, with and without the substrate, fails to 
elicit binding to the column. The protein is loaded in low ionic strength 
buffers.

Does anybody have a preference of gel type for  for this type of column? Are 
there any tricks to aid binding to the column?

Many thanks for any help

Chris
-- 
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Chris Snook, Ph.D.,        office  (573)-884-2302
Dept. of Biochemistry,     FAX     (573)-884-4812
117 Schweitzer Hall,
University of Missouri,
Columbia, MO 65211, USA
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