[ccp4bb]: phase combination problem

["thore@embl-heidelberg.de" <thore@embl-heidelberg.de>]

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Dear all,

I have a first set of phase from a MAD experiment at the selenium edge from
a crystal indexed in p41212 with the respective cell parameter 78.6 78.6
102.6 90 90 90 and 1 molecule per assymetric unit. This set of phase gives
a map in which almost 50%  of the backbone of the protein can be built but
without assigning the sequence (I have tried Solve 2.03 and Sharp. Both
programs gave me very similar phases which gave very similar maps).
I also have a xenon derivative from crystals in the same condition but
where the indexation cannot be done in p41212, this time it's p212121 with
the following cell parameter 78.4 79.5 101.1 90 90 90 and 2 molecules per
assymetric unit. This derivative also gives a set of phase but not enough
to give interpretable map.
I tried to combine the two sets of phases with the program Dmmulti. The
rotation matrix that I used to move from the p41212 crystal to the p212121
crystal is the one necessary to pass from the position of the molecule in
the p41212 crystal (obtained by molecular replacement with the partial
model) to the 2 positions of the two molecules in the p212121 crystal (also
obtained by molecular replacement with the same partial model and with the
same program molrep). Unfortunately it didn't work very well. Was I wrong
with the rotation matrix I used ? or is it another reason ?
Is Dmmulti the only program to try to do some phase combination ?

Thanks a lot

stephane

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Stephane THORE
Suck group
Structural Biology and Biocomputing program
EMBL
1, meyerhofstrasse
69117 Heidelberg

Tel  49-6221 387494
Fax  49-6221 387519
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