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[ccp4bb]: Q: disulphide reduction in protein structures
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Sorry this is not a CCP4 question.
Can buried (i.e. small or no solvent accessible surface area) disulphide
bonds be reduced to cysteines in general? I have a structure in which 2
cysteines are in the right position to form a S-S bond but the electron
density shows otherwise. My protein was purified in reducing
conditions. However, from other evidences (structure homology), these
should form S-S. Does anyone know of any published structure that can
shed light on my puzzle? Thanks a lot.
Yu Wai CHEN, Ph.D. .................. email:firstname.lastname@example.org
Centre for Protein Engineering, tel:+44-(0)1223-402148
MRC Centre, Hills Rd, Cambridge CB2 2QH, UK fax:+44-(0)1223-402140
WWW homepage: http://www.mrc-cpe.cam.ac.uk/~ywc