Re: Freezing

[Katjusa Brejc <katjusa@nki.nl>]

***  For details on how to be removed from this list visit the  ***
***    CCP4 home page http://www.dl.ac.uk/CCP/CCP4/main.html    ***



HI Swami,

what about trying freezing crystals in some other salt like sodium format?
There is a paper published where the authors reported freezing crystals in
6 M sodium format (crystals grew from 3.5 M sodium format; Lee et al
(1998), Nature 391:859-865).


Sincerely,


Katjusa


*****************************
Katjusa Brejc
Netherlands Cancer Institute
Dept. Molecular Carcinogenesis
Plesmanlaan 121
1066 CX AMSTERDAM
The Netherlands
*****************************

> Dear :
> 
> I have got a crystal and the condition is:
> 
> 100 mM Tris-HCl, pH 7.5
> 150 mM Nacl
> 20 mM  Ca Cl2
> 
> (The crystal dissolves in conventional method of freezing, time course
> transfer in artificial mother liquor + 5%, 10%, 15%, 20%, 25% glycerol, MPD,
> PEG400).  I am looking for suggestions/advice for freezing crystals grown in
> pure salt environment.  Any tips?
> 
> With regards,
> Swami
> 
>   ****************************************************************************
>   Dr. Kunchithapadam Swaminathan		Phone: 	(65) 873 2173 (H)
>   Institute of Molecular Agrobiology			(65) 872 7408 (W)
>   The National University of Singapore		Fax:	(65) 872 7007 (W)
>   1 Research Link, Singapore 117604		Email:	nathan@ima.org.sg
> 
>   Web:	www.ima.org.sg/research/struct_bio/struct_bio.html
>   ****************************************************************************
> 
>