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Re: Freezing

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For fun, try taking an old lysozyme crystal and mounting it in a loop.
Then look at it under a microscope and see what happens. I think that "dry
mounting" (without any mother liquor) is a bit risky. You can dry the
crystal out VERY quickly. 

One trick I use is warming up N-Paratone and mixing with water. I let this
settle over-night and use the oil fraction for cryoprotection. I was
expecting ice rings the first time round, but it works wonders with my
crystals! Just try anything!!!


Paul Hubbard
Department of Biochemistry
Medical College of Wisconsin
8701 Watertown Plank Rd.
Wisconsin. 53226

Tel: 414-456-4305
Fax: 414-456-6510