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Pardon me for asking a non-CCP4 related question here. But, I really feel
somebody out there can help me solve my problem.
I have a very-well expressed protein. It is fine at both 4c and room
temperature (rt) as long as its concentration is below 1 mg/ml. If I
concentrate the protein to 4 mg/ml or even 20 mg/ml or so, they are still
fine at 4c. However, the protein solutions become very cloudy if I
transfer them to rt or 16c. The protein solutions will turn to clear
again after they are put back to 4c for a while.
Is there anyone who has experience this before ? What could be the
problem ? Does it mean something wrong with the protein folding ? Is
there any suggestion ?
Your help is really appreciated. Thanks in advance.