[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]
Re: Temperature factors and Wilson Plots
*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.dl.ac.uk/CCP/CCP4/main.html ***
Andy Gulick wrote:
> *** For details on how to be removed from this list visit the ***
> *** CCP4 home page http://www.dl.ac.uk/CCP/CCP4/main.html ***
> I have just solved a structure with MAD phasing and am trying to refine
> with REFMAC
> rather than TNT (which is normally used in this lab). The model is
> fairly consistant with related proteins (so I know there are no gross
> errors) and the data are good to 2.4 A.
> I ran the data through TRUNCATE, UNIQUE, and then began my refinement
> with REFMAC.
I believe that average B factors given for structures are more often
too low than too high.. If the data peters out at 3A it is very
difficult to get any sensible estimate of a Wilson plot gradient, but it
is most likely that a true Av_B should be in the range 80-100, rather
than 10 as is sometimes given!
REFMAC values should reflect the gradient of the Wilson plot, and they
do seem to do so quite well in your case.