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Re: Temperature factors and Wilson Plots
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> As yet, this all seems unremarkable. What surprised me, however, was
> that the
> temperature factors for the refined structure were quite high. They
> average temperature
> factor is 65 and ranges from 40 to 130. I browsed through the old posts
> to
> this list and found a suggestion that your protein atom temperature
> factors should
> approximate your B from a Wilson plot. I then looked at my truncate
> output
> and found the Wilson B value was 56.
To comfort you, here are my figures of a recent structure solved, which
is very similar to yours:
data to 2.5 A
R/R-free = 28/34
average B = 62
B estimated from Wilson = 59
Wai
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Yu Wai CHEN, Ph.D. .................. email:ywc@mrc-lmb.cam.ac.uk
Centre for Protein Engineering, tel:+44-(0)1223-402148
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