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> a) What conditions have people succesfully used to overcome
> twinning in crystallization?
I've often had success with low concentrations of organics and
detergents for slightly altering crystal growth. Examples are 1-3% MPD or
5-15mM BOG. This has resulted in less crystal intergrowth and reduction
in twinning, although it may not help severe problems. The concentrations
are low enough to not interfere with overall crystal growth in most cases.
Best of luck,
Jeffrey A. Speir, Ph.D.
The Scripps Research Institute
Dept. of Molecular Biology - BCC206
10550 North Torrey Pines Road
La Jolla CA 92037
Phone: (858) 784-2238
Fax: (858) 784-2980
- From: Titia Sixma <firstname.lastname@example.org>