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[ccp4bb]: soluble aggregation of proteins

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Dear all,

We are expressing proteins here in E. Coli in the pMALp2 vector as
maltose-binding fusion proteins. This is a periplasmic expression and we
have found that our three proteins in the 22-38 KDa range all aggregate
in solution. 

Any suggestions from your experience to treat this type of problem?



Janet Moloney, Division of Structural Biology,
Wellcome Trust Centre for Human Genetics,
University of Oxford, Roosevelt Drive, 
Oxford OX3 7BN, U.K.

Tel: 0044(0)1865 287551	   Fax: 0044(0)1865 287547