[ccp4bb]: Re: Chemical modification of cystein residues

[Evangelos Christodoulou <echristo@biology.db.uoa.gr>]

on 11/4/00 12:20, Karsten Niefind at Karsten.Niefind@uni-koeln.de wrote:

> ***  For details on how to be removed from this list visit the  ***
> ***    CCP4 home page http://www.dl.ac.uk/CCP/CCP4/main.html    ***
> 
> Dear collegues,
> 
> does anybody know references about chemical modification of
> cystein sulfhydryl groups of proteins to improve subsequent
> crystallization? Any hints are welcome!

I have used iodoacetamide to 'block' free cystein residues.


>From "Gel Electrophoresis of Proteins A Practical Approach"
ISBN 0-19-963075-5
Edited by B. D. Hames and D. Rickwood
pages 353-4 (see attached picture)

Hope this helps.
Van



-- 
Evangelos Christodoulou
University of Athens, Greece
If Che Guevara was alive he'd use a Mac
-the only revolutionary-friendly platform

Iodoacetamide.pic