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[ccp4bb]: Re: Chemical modification of cystein residues

on 11/4/00 12:20, Karsten Niefind at Karsten.Niefind@uni-koeln.de wrote:

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> Dear collegues,
> does anybody know references about chemical modification of
> cystein sulfhydryl groups of proteins to improve subsequent
> crystallization? Any hints are welcome!

I have used iodoacetamide to 'block' free cystein residues.

>From "Gel Electrophoresis of Proteins A Practical Approach"
ISBN 0-19-963075-5
Edited by B. D. Hames and D. Rickwood
pages 353-4 (see attached picture)

Hope this helps.

Evangelos Christodoulou
University of Athens, Greece
If Che Guevara was alive he'd use a Mac
-the only revolutionary-friendly platform