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[ccp4bb]: SeMet incorporation erratum
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Dear All
Sean Nair has pointed out typos in the protocol I posted:
1) I added 1ml of 4.2 g/l FeSO4 per litre
2) I added 100ml of 4% glucose (w/v) per litre
both sterile filtered
- many apologies for that
cheers
Martyn
---------- Forwarded message ----------
Date: Wed, 05 Jul 2000 08:10:29 +0100
From: Sean Nair <snair@eastman.ucl.ac.uk>
To: martyn@cryst.bioc.cam.ac.uk
Subject: SeMet labelling protocol
Dear Martyn Simmons,
I was interested in trying out the protocol that you put on the ccp4
bulletin board but I wanted to check two thing out with you first. 1)
you added 1ml of FeIIS04 what was the concentration?
2) Is the concentration of glucose used 10ml of a 4% (w/v) and not 40%
(w/v). I only ask because it is much lower than I am used to using in M9.
Thanks for your help
Sean