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Re: [ccp4bb]: cis-glycine!
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Liu Lijun wrote:
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> Hello all,
> I am now refining a structure at 1.9A. A glycine residue was too stubborn
> to be arranged. 2Fo-Fc, Fo-Fc and omit maps all showed that the
> conformation of the glycine should be much different from the normal one.
> After adjusting it to the positions where I thought best fitting the maps,
> I ran the PROCHECK, and the results proved and figured it as cis-.
> * cis-peptide: GLY 11
> Now the problem was that I could not refined the "cis-" glycine together
> since it would be automatically changed to the normal conformation. In the
> case of cis-proline, I think I know how to deal with, but I have no idea
> here, so did anyone experience this or could you give me any suggestions???
> LIU Lijun
The key word in PROTIN is CISP -EPTIDE..
not CISP -roline..
Of course most CIS peptides ARE prolines but there is no restriction in