[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]
Re: [ccp4bb]: cis-glycine!
*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.dl.ac.uk/CCP/CCP4/main.html ***
Liu Lijun wrote:
>
> *** For details on how to be removed from this list visit the ***
> *** CCP4 home page http://www.dl.ac.uk/CCP/CCP4/main.html ***
>
> Hello all,
>
> I am now refining a structure at 1.9A. A glycine residue was too stubborn
> to be arranged. 2Fo-Fc, Fo-Fc and omit maps all showed that the
> conformation of the glycine should be much different from the normal one.
> After adjusting it to the positions where I thought best fitting the maps,
> I ran the PROCHECK, and the results proved and figured it as cis-.
> -----------
> * cis-peptide: GLY 11
> -----------
>
> Now the problem was that I could not refined the "cis-" glycine together
> since it would be automatically changed to the normal conformation. In the
> case of cis-proline, I think I know how to deal with, but I have no idea
> here, so did anyone experience this or could you give me any suggestions???
>
> thanks
>
> LIU Lijun
The key word in PROTIN is CISP -EPTIDE..
not CISP -roline..
Of course most CIS peptides ARE prolines but there is no restriction in
the code..
Eleanor Dodson