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[ccp4bb]: disappeared crystal...
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I'm sorry this is not a direct ccp4 question rather about
crystallization. I was setting up crystallization trials using
Ammonium sulfate screen (Hampton Research) for my protein
(sitting drop method). After finishing the setup at room
temperature, I sealed the plate with adhesive tape after
(~) 15 minutes. Before putting the plates at low temp. I just
scaned the plate under microscope. I saw big crystals in two
wells (within 20 min). When I fixed the camera and ready to
take a snap of the crystal I could not see anything in the same
well, all crystal disappeared suddenly. The dish was always at
room temperature and everthing happened within 30 to 45 minutes.
Now I have following questions.
1) Is somebody have experience like this ?
2) I tried to set the same condition again but, I could not
get any crystal? any suggestions ?
3) Any literature or paper regarding this type of problem?
4) or it is just a salt crystal ? :(
Any comments or suggestion are highly appreicated.
subramanian karthikeyan, email@example.com
Harvard Institutes of Medicine http://www.bidmc.harvard.edu
4 Blackfan Circle
Boston, Tel:- +1-617-667 0068
MA, 02115 USA. Fax:- +1-617-975 5243