[ccp4bb]: Re: Protein & salt

[Arjan Snijder <a.snijder@chem.rug.nl>]

If you need such high salt concentrations to keep this protein soluble
why not try salting in for growing crystals?

Regards Arjan
 

tommy wang wrote:

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I have a protein that requires 0.5 to 1M NaCl to be soluble at 10mg/ml. My
question is whether I should bother screening this protein and whether there
are any protein crystals grown using protein buffers at such high salt
concentrations? I am aware that ammonium sulfate is used at high
concentrations to grow many crystals but are there crystals successfully
grown from proteins that require high salt concentrations to be soluble?
Please share your experience. Thanks

Tommy Wang

-- 
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Arjan Snijder
Lab. of Biophysical Chemistry
University of Groningen
Nyenborgh 4
9747 AG Groningen
tel +31 (0) 50 3634396
fax +31 (0) 50 3634800
a.snijder@chem.rug.nl
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